Metabolic Labeling (SILAM)
SILAM refers to labeling an entire rodent with heavy isotopes for quantitative proteomic tissue analysis. Labeling an entire proteome with heavy isotopes in vivo generates an ideal standard for quantitative proteomics. When a heavy labeled proteome is mixed with an unlabeled proteome then digested, every unlabeled peptide identified by the mass spectrometer can be quantified by its corresponding heavy peptide.The advantage of metabolic labeling over in vitro tagging techniques is that the heavy and unlabeled samples are mixed before sample preparation, preventing variability between preparations from distorting the quantification results. This is especially important when extensive sample preparation (e.g. isolation of an organelle) is required. In SILAM, the rodent food is altered to contain heavy lysine or 15N spirulina as the only protein source.The heavy tissues are used as internal standards for quantitative proteomic analysis of basic mammalian physiology and animal models of disease. All these products can be found in our Stable Isotopes for Mass Spectrometery catalogue. The below items are specific for these product areas and there are specific application notes that may be helpful and are just generally good reads.
If you have any questions or wish to discuss your needs then please don’t hesitate to contact us.
Cambridge Isotope Laboratories, Inc. is pleased to offer intact stable isotope-enriched mouse tissue for use in SILAM: Mouse Express® L-Lysine (13C6, 97%) Mouse Tissue and Mouse Express® (15N, 94%) Mouse Tissue
- Stable Isotope Labeling in Mammals (SILAM)
- 15N Stable Isotope Labeling Data Analysis
- Stable Isotope Labeling in Mammals with 15N Spirulina (Application Note 24)
- Targeted LC-SRM/MS Quantification of Mammalian Synaptic Proteins with MouseExpress® Brain Tissue (Application Note 27)
- Analysis of Tyrosine Kinase Signaling in Human Cancer by Stable Isotope Labeling with Heavy Amino Acids in Mouse Xenografts Utilizing MouseExpress® Lysine 13C6 Mouse Feed (Application Note 32)
- Protein Turnover
- Determining Protein Turnover in Fish D7 Leucine (Application Note 29)
- SILAM Literature References
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